A large discoidal lipoprotein present in only one of two closely related crayfish

作者：Stieb Stefanie; Hoeger Ulrich^*; Schenk Sven

来源：Journal of Comparative Physiology B-Biochemical Systems and Environmental Physiology, 2008, 178(6): 755-765.

DOI：10.1007/s00360-008-0266-8

• crayfish
• lipoprotein
• density gradient ultracentrifugation
• electron microscopy
• MALDI-TOF-TOF

The hemolymph lipoproteins of two European freshwater crayfish, Astacus astacus and Astacus leptodactylus, were isolated and characterized. The former species possesses two sex-independent lipoproteins, which can be related to the formerly described high-density lipoprotein (HDL)/beta-glucan binding protein and very high-density lipoprotein/clotting protein from other crustaceans. The latter species, however, contains an additional third lipoprotein with a unique structure. It is a large discoidal HDL with a diameter of 42 nm, a thickness of 7 nm and a density of 1.1 g/ml. SDS-PAGE revealed two different apolipoproteins with molecular masses of 240 and 85 kDa, respectively, arranged in a 1:1 stoichiometry as judged from cross linking experiments. The lipid content of this lipoprotein was 67%, far higher than in every other crustacean lipoprotein described so far. The native molecular mass of this HDL-type lipoprotein was estimated to be about 930 kDa. The lipid content of the other lipoproteins ranged between 25 and 30% for the HDL/beta-glucan binding protein and 6-8% for the VHDL/clotting protein.

• 出版日期2008-8