Novel Essential Gene Involved in 16S rRNA Processing in Escherichia coli

作者：Kurata Tatsuaki; Nakanishi Shinobu; Hashimoto Masayuki; Taoka Masato; Yamazaki Yukiko; Isobe Toshiaki; Kato Jun ichi^*

来源：Journal of Molecular Biology, 2015, 427(4): 955-965.

DOI：10.1016/j.jmb.2014.12.013

Biogenesis of ribosomes is a complex process mediated by many factors. While its transcription proceeds, ribosomal RNA (rRNA) folds itself into a characteristic three-dimensional structure through interaction with ribosomal proteins, during which its ends are processed. Here, we show that the essential protein YqgF, a RuvC family protein with an RNase-H-like motif, is involved in the processing of pre-16S rRNA during ribosome maturation. Indeed, pre-16S rRNA accumulated in cells of a temperature-sensitive yqgF mutant (yqgF(ts)) cultured at a non-permissive temperature. In addition, purified YqgF was shown to process the 5' end of pre-16S rRNA within 70S ribosomes in vitro. Mass spectrometry analysis of the total proteins in the yqgF(ts) mutant cells showed that the expression of genes containing multiple Shine Dalgarno-like sequences was observed to be lower than in wild type. These results are interpreted to indicate that YqgF is involved in a novel enzymic activity necessary for the processing of pre-16S rRNA, thereby affecting elongation of translation.

• 出版日期2015-2-27