Accelerated Fmoc solid-phase synthesis of peptides with aggregation-disrupting backbones

作者：Huang, Yi Chao; Guan, Chao Jian; Tan, Xiang Long; Chen, Chen Chen; Guo, Qing Xiang; Li, Yi Ming^*

来源：Organic and Biomolecular Chemistry, 2015, 13(5): 1500-1506.

DOI：10.1039/c4ob02260b

• 1 ,4-Dioxane
• Accelerated solid
• Air baths
• Protein segments
• Solid phase synthesis
• Synthetic protocols
• Ubiquitin

In this work, we describe an accelerated solid-phase synthetic protocol for ordinary or difficult peptides involving air-bath heating and amide protection. For the Hmsb-based backbone amide protection, an optimized acyl shift condition using 1,4-dioxane was discovered. The efficiency and robustness of the protocol was validated in the course of preparation of classical difficult peptides and ubiquitin protein segments.

• 出版日期2015
• 单位合肥工业大学; 清华大学; 中国科学技术大学