Diagnosis of Mycoplasma genitalium by MgPa and rRNA 16S gene amplification

作者：Fernandez Molina Carmen^*; Rodriguez Preval Nadia; Rodriguez Gonzalez Islay; Agnese Latino Maria; Antonio Rivera Tapia Jose; Ayala Rodriguez Idalia

来源：Salud Publica de Mexico, 2008, 50(5): 358-361.

• diagnosis
• Mycoplasma genitalium
• PCR
• Cuba

Objective. Mycoplasma genitalium has been associated with nongonococcal urethritis (NGU). Diagnosis by PCR has become the primary detection method for this organism. Thus, diagnosis by DNA amplification using the PCR technique should be utilized. Material and Methods. GMF/GMR and MgpF/MgpR primer pairs, complementary to the M. genitalium 16S rRNA and MgPa genes, respectively, were selected. Specificity and sensibility assays were conducted and clinical samples were studied. Results. The PCR with each primer pair was specific only for M. genitalium, and the sensibility was higher with the GMF/GMR primers. In the study of 34 clinical samples, 18,5% were positive for M. genitalium, with more positive samples when the MgpF/MgpR primers were used. Conclusions. DNA amplification by PCR should be applied in clinical practice to the diagnosis of M. genitalium in patients with NGU should using.

• 出版日期2008-10
• 单位河北医科大学