Anti-inflammatory effects of Perilla frutescens leaf extract on lipopolysaccharide-stimulated RAW264.7 cells

作者：Huang Bee Piao; Lin Chun Hsiang; Chen Yi Ching; Kao Shao Hsuan^*

来源：Molecular Medicine Reports, 2014, 10(2): 1077-1083.

DOI：10.3892/mmr.2014.2298

• Perilla frutescens
• pro-inflammatory mediators
• macrophage
• nuclear factor-kappa B

Perilla leaves are widely used in Chinese herbal medicine and in Japanese herbal agents used to treat respiratory diseases. This study aimed to investigate the anti-inflammatory effects and the underlying mechanisms of Perilla frutescens leaf extract (PLE). Murine macrophage RAW264.7 cells were used as a model. Cell viability and morphological changes were studied by the MTT assay and microscopy. mRNA expression of pro-inflammatory mediators was assessed by both semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and quantitative (q) RT-PCR. Nitric oxide (NO) and prostaglandin E-2 (PGE(2)) production were analyzed by the Griess test and sandwich enzyme-linked immunosorbent assay (ELISA), respectively. The activation of kinase cascades was studied by immunoblotting. Our findings showed that PLE slightly affects cell viability, but alleviates LPS-induced activation of RAW264.7 cells. Furthermore, PLE significantly reduced the LPS-induced mRNA expression of the interleukin (IL)-6, IL-8, tumor necrosis factor-alpha (TNF-alpha), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), genes in a dose-dependent manner. In addition, PLE reduced NO production and PGE(2) secretion induced by LPS. PLE also inhibited activation of mitogen-activated protein kinases (MAPKs), increased the cytosolic I kappa B alpha level, and reduced the level of nuclear factor (NF)-kappa B. Taken together, these findings indicate that PLE significantly decreases the mRNA expression and protein production of pro-inflammatory mediators, via the inhibition of extracellular-signal-regulated kinase (ERK)112, c-Jun N-terminal kinase (JNK), p38, as well as NF-kappa B signaling in RAW264.7 cells stimulated with LPS.

• 出版日期2014-8