Proteome analysis of human cerebrospinal fluid as a diagnostic biomarker in patients with meningioma

作者：Kim Jae Ho; Lee Sang Kwang; Yoo Yong Cheol; Park Nam Hyun; Park Dan Bi; Yoo Jong Shin; An Hyun Joo; Park Young Mok; Cho Kyung Gi^*

来源：MEDICAL SCIENCE MONITOR, 2012, 18(11): BR450-BR460.

DOI：10.12659/MSM.883538

• meningioma
• human cerebrospinal fluid
• proteome
• biomarker
• brain tumor

Background: To identify meningioma-specific proteins, cerebrospinal fluid (CSF) from 4 patients with a meningioma and 4 patients with a non-brain tumorous lesion were analyzed. %26lt;br%26gt;Material/Methods: Two-dimensional electrophoresis and electrospray quadrupole time-of-flight tandem mass spectrometry analyses revealed 10 unique spots, containing 11 independent proteins (spot #2 and #4 each contained 2 proteins and spot #3 was not identified) were evident in CSF associated with human meningioma: serum albumin precursor (3 different isoforms), Apolipoprotein E (Apo E), Apolipoprotein J precursor (Apo J), Transthyretin precursor (TTR), Prostaglandin D2 synthase 21kDa (PTGDS), proapolipoprotein, Chain D hemoglobin Ypsilanti, alpha-1-antitrypsin (AAT), and beta-2-microglobulin precursor (beta 2M). %26lt;br%26gt;Results: The contents of Apo E, Apo J and AAT were increased, while PTGDS, TTR and beta 2M were decreased. %26lt;br%26gt;Conclusions: The results observed by 2-dimensional electrophoresis were verified by Western blot analysis. The unique proteins may represent possible candidate biomarkers of meningioma.

• 出版日期2012-11