Acetylsalicylic acid supplementation improves protein utilization efficiency while vitamin E supplementation reduces markers of the inflammatory response in weaned pigs challenged with enterotoxigenic E. coli

作者：Kim Jae Cheol^*; Mullan Bruce P; Black John L; Hewitt Robert J E; van Barneveld Robert J; Pluske John R

来源：Journal of Animal Science and Biotechnology, 2016, 7(1): 58.

DOI：10.1186/s40104-016-0118-4

• Acetylsalicylic acid
• E. coli infection
• PGE(2)
• Vitamin E
• Weaner pigs

Background: This experiment was conducted to test the hypothesis that vitamin E (Vit E) and acetylsalicylic acid (ASA), a cyclooxygenase-2 (COX-2) inhibitor, will additively reduce the production of the immunosuppressive molecule prostaglandin E-2 (PGE(2)) and hence reduce inflammatory responses in weaner pigs experimentally infected with an enterotoxigenic strain of E. coli.
Methods: The experiment was conducted in a research facility with 192 individually-housed male weaner pigs (Landrace x Large White) weighing 6.6 +/- 0.04 kg (mean +/- SEM). The pigs were experimentally infected with an enterotoxigenic strain of E. coli and were allocated to a 2 x 3 factorial design with the respective factors being without and with 125 ppm ASA and three levels of Vit E supplementation (50, 100 or 200 IU/kg diet, dl-alpha-tocopheryl acetate).
Results: Acetylsalicylic acid supplementation improved average daily gain (P < 0.05) and tended to improve feed: gain ratio (P < 0.10) during the first 14 d after weaning. Acetylsalicylic acid supplementation also improved (P < 0.001) amino acid utilization efficiency (as assessed by plasma urea level) and tended to decrease (P < 0.10) PGE(2) production in the liver without affecting small intestinal histology and tight junction protein mRNA expression in the jejunal epithelium. Vitamin E supplementation greater than 100 IU/kg diet sustained both the plasma Vit E concentration (P < 0.001) and plasma haptoglobin content (P < 0.001) after weaning. However, there was no additive effects of the combined supplementation of ASA and Vit E on performance, intestinal barrier function and inflammatory responses of weaned pigs.
Conclusions: Although ASA and vitamin E improved amino acid utilization efficiency and reduced acute inflammatory responses, ASA and vitamin E did not additively reduce production of PGE(2) and inflammatory responses in weaner pigs experimentally infected with an enterotoxigenic strain of E. coli.

• 出版日期2016-10-3