An important role for secreted esterase in disease establishment of the wheat powdery mildew fungus Blumeria graminis f. sp tritici

作者：Feng Jie; Wang Feng; Hughes Geoff R; Kaminskyj Susan; Wei Yangdou^*

来源：Canadian Journal of Microbiology, 2011, 57(3): 211-216.

DOI：10.1139/W10-120

• serine esterase
• cutinase
• pathogenesis
• powdery mildew
• wheat

The activity of esterase secreted by conidia of wheat powdery mildew fungus, Blumeria graminis f. sp. tritici, was assayed using indoxyl acetate hydrolysis, which generates indigo blue crystals. Mature, ungerminated, and germinating conidia secrete esterase(s) on artificial media and on plant leaf surfaces. The activity of these esterases was inhibited by diisopropyl fluorophosphate, which is selective for serine esterases. When conidia were inoculated on wheat leaves pre-treated with diisopropyl fluorophosphate, both appressorial germ tube differentiation and symptom development were significantly impaired, indicating an important role of secreted serine esterases in wheat powdery mildew disease establishment.

• 出版日期2011-3
• 单位Saskatoon; Saskatchewan