Adriamycin dose and time effects on cell cycle, cell death, and reactive oxygen species generation in leukaemia cells

作者：Savatier Julien; Rharass Tareck; C**** Christophe; Gbankoto Adam; Vigo Jean; Salmon Jean Marie; Ribou Anne Cecile^*

来源：Leukemia Research, 2012, 36(6): 791-798.

DOI：10.1016/j.leukres.2012.02.017

• Adriamycin
• Cell cycle
• Multiparametric analysis
• Polyploid cells
• Reactive oxygen species
• Apoptosis

We investigate the relative importance of the different mechanisms of Adriamycin, an anthracycline, and their interrelations, in particular the link between cell cycle arrest, cell death, and generation of reactive oxygen species (ROS) that is suspected to be the origin of cardiotoxic side-effects. We introduced a lifetime fluorescence based technology and used videomicrofluorometry, two efficient analytical methods. We show that depending on the doses and time after incubation, ADR will not reach the same compartments (nucleus, mitochondria, cytosol) in the cells, having consequences on the production of ROS, growth arrest pathways and cell death pathways.

• 出版日期2012-6