The immunostimulatory effect of saponin immersion against Vibrio alginolyticus in swimming crab Portunus trituberculatus

作者：Ng'ambi, Jonas Wiza; Li, Ronghua; Mu, Changkao; Song, Weiwei; Wang, Chunlin^*

来源：Aquaculture International, 2017, 25(4): 1667-1678.

DOI：10.1007/s10499-017-0139-9

• Quillaja saponin
• Portunus trituberculatus
• Vibrio alginolyticus
• Antioxidant enzymes
• Immune gene expression

Swimming crab Portunus trituberculatus immersed in 1 mg L-1 saponin showed a significant increase in total hemocyte, hyaline, and granular cell counts compared to the control (p < 0.05). Superoxide dismutase, glutathione peroxidase, and phagocytic activities increased significantly compared to the control. Heat shock protein (HSP) 60 and immune gene transcripts (crustin, lectin, prophenoloxidase, and CuZnSOD) increased significantly (p < 0.05) to the highest levels by 48 h of immersion and then declined sharply at 72 h. Although immersion of P. trituberculatus in 1 mg L-1 saponin may enhance its immune response and resistance against Vibrio alginolyticus infection, the results of this study suggest that immunosuppression may take place when the crabs are immersed longer than 48 h.

• 出版日期2017-8
• 单位宁波大学