科研之友
微信
新浪微博
Facebook
分享链接
摘要
Lettuce (Lactuca sativa L.), a leafy vegetable belonging to Asteraceae family, possesses sedative-hypnotic, anticonvulsant, anti-inflammatory, antinociceptive, anticancer, and antioxidant properties. Currently, only a few microsatellite markers have been developed from lettuce, which hindered the genetic research. In the present study, EST-SSRs were developed and characterized from 81,518 expressed sequence tags of lettuce. The results showed that tri-nucleotide motif (40.789%) was the most abundant, followed by di-nucleotide (33.109%) and hexa-nucleotide repeats (16.295%). Among 100 primer pairs designed according to SSR-containing sequences, a set of 35 could give out reproducible and clear amplification products, and 15 were polymorphic. These novel microsatellite markers were used to evaluate genetic diversity of lettuce and its related species. The number of alleles per locus ranged from 3 to 11, with a mean value of 6.2. The expected heterozygosity (HE) and observed heterozygosity (HO) ranged from 0.5141 to 0.8346 and 0.0484 to 0.6000, respectively. Eleven could be transferable to three related species, including Artemisia argyi H. Lev. & Vaniot, Erigeron annuus (L.) Pers., and Dendranthema morifolium (Ramat.) Tzvel. These novel microsatellite markers will not only enrich current resource of genetic markers, but also be useful in the following cultivar identification, germplasm evaluation, and marker-assisted selection of L. sativa and related species.
- 出版日期2017-11
- 单位黄冈师范学院; 武汉市农业科学技术研究院蔬菜科学研究所
