Up-Regulation of A-Kinase Anchoring Protein in the Nucleus Tractus of Solitarius of Rats by Intraperitoneal Lithium

作者：Hwang Y J; Lee J Y; Lee J H; Jahng J W^*

来源：International Journal of Pharmacology, 2010, 6(3): 208-215.

• Lithium
• nucleus tractus of solitarius
• proteomics
• A-kinase anchoring protein
• cAMP response element-binding protein

We have examined the proteome patterns of the Nucleus Tractus of Solitarius (NTS) of rats shortly after an intraperitoneal injection of lithium chloride at a conventional dose widely used as unconditioned stimulus inducing the formation of conditioned taste aversion. The intraperitoneal lithium induced an up-regulation of A-Kinase Anchoring Protein (AKAP), which binds to cAMP-dependent protein kinase A (PKA) and regulates the phosphorylation of various proteins implicated in synaptic plasticity and memory consolidation. Expressions of cAMP/PKA signaling related genes and proteins in the NTS were analyzed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Western blot and phosphorylation of cAMP response element-binding protein (CREB) was increased in the brainstem NTS by the intraperitoneal lithium. These results suggest that up-regulation of AKAP and CREB activation may be involved in the lithium-induced c-fos expression in the rat NTS. In addition, tentative implications of tight junction protein, cadherin protein and acetyl-Co A carboxylase in the lithium-induced synaptic plasticity and memory formation are discussed.

• 出版日期2010-6