Abnormal neural crest innervation in Sox10-Venus mice with all-trans retinoic acid-induced anorectal malformations

作者：Suzuki Ryota; Miyahara Katsumi; Murakami Hiroshi; Doi Takashi; Lane Geoffrey J; Mabuchi Yo; Suzuki Nobuharu; Yamataka Atsuyuki; Akazawa Chihiro^*

来源：Pediatric Surgery International, 2014, 30(2): 189-195.

DOI：10.1007/s00383-013-3452-z

• Retinoic acid
• Anorectal malformation
• Neural crest innervation
• Anal sphincter
• Sox10
• Transgenic mouse

Despite technical advances in the surgical/medical care of anorectal malformation (ARM), persistent unsatisfactory postoperative bowel habit has been attributed to histopathologic abnormalities of the distal rectum/pouch (DRP) and hypoplasia of anal sphincter muscles (ASM). We used Sox10-Venus mice with ARM induced by all-trans retinoic acid (ATRA) to investigate neural crest cell (NCC) innervation in the DRP and ASM. %26lt;br%26gt;Pregnant Sox10-Venus mice were administered single doses of 50, 70, or 100 mg/kg of ATRA on embryonic day 8.5 (E8.5) then sacrificed on either E16.5 or E19.5. Bowel specimens comprising the anorectum were examined using fluorescence microscopy without immunohistochemical staining (FMIS). Anti-PGP9.5 was used to delineate ganglion cells and anti-SMA for smooth muscles. %26lt;br%26gt;The appropriate dose of ATRA for inducing ARM was 50 mg/kg. Under FMIS, all ARM embryos (n = 5; all high type; 3 male:2 female) had less NCC innervation with thick Venus-positive nerve fibers in the DRP compared with normal embryos (n = 8); there was abnormal NCC innervation in the DRP and absent ASM in ARM mice. %26lt;br%26gt;We are the first to delineate abnormal enteric nervous system innervation in the DRP of ARM mice without using immunohistochemical staining techniques thus allowing specimens to be examined without any distortion.

• 出版日期2014-2