科研之友
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摘要
A novel fluorescence method for the determination of guanine was developed based on the fluorescence enhancement of Cu2+-nuclear fast red complex in the presence of guanine in Tris-HCl buffer. The complex of Cu2+ with nuclear fast red resulted in a dramatic quenching of the fluorescence intensity. Nuclear fast red were dissociated from the complex with the addition of guanine due to the strong interaction between guanine and Cu2+, which caused the fluorescence enhancement The enhanced fluorescence intensity was well proportional to the concentration of guanine in the range of 4.96 x 10(-8)-1.09 x 10(-6) mol/L with the limit of detection 1.9 x 10(-8) mol/L. The method has been applied successfully to the determination of guanine in serum and DNA samples, and the recoveries were from 96.0% to 104.8%.
