Phytochemical distinction between Pelargonium sidoides ("Umckaloabo") and P. reniforme through H-1-NMR and UHPLC-MS metabolomic profiling

作者：Viljoen Alvaro M^*; Zhao Jianping; Sandasi Maxleene; Chen Weiyang; Khan Ikhlas A

来源：Metabolomics, 2015, 11(3): 594-602.

DOI：10.1007/s11306-014-0722-2

• H-1-NMR
• UHPLC-MS
• Metabolomics
• Pelargonium sidoides
• Pelargonium reniforme
• Multivariate data analysis

Two Pelargonium species which are indigenous to South Africa; P. sidoides DC and P. reniforme Curt., are highly valued traditional remedies used primarily for the treatment of upper respiratory tract infections. The ethnobotanical value of P. sidoides has led to the commercialisation of herbal medicinal products such as Umckaloabo(A (R)) that are marketed internationally. Although the two species can be distinguished by leaf shape and flower colour, the rhizomes which are used for preparation of the commercial extracts are morphologically similar. Non-targeted H-1-NMR and UHPLC-MS metabolomic approaches were applied to differentiate P. sidoides and P. reniforme extracts. qNMR was used for the quantitative comparison of the marker constituents occurring in both species. Both H-1-NMR and UHPLC-MS in combination with multivariate algorithms revealed a chemical distinction between the two species. Umckalin was identified as the marker compound in P. sidoides (0.0012-0.2760 % (w/w)) by UHPLC-MS, which occurred in trace quantities in P. reniforme (0-0.0016 % (w/w)). Conversely, coumarins such as scopoletin, isofraxoside and a scopoletin isomer were reported to occur in relatively higher levels in P. reniforme compared to P. sidoides.

• 出版日期2015-6