Applied multiplex allele specific PCR to detect second-line drug resistance among multidrug-resistant tuberculosis in China

作者：Liang, Ya-ping; Chen, Yan; Xiao, Tong-yang; Xia, Qiang; Liu, Hai-can; Zhao, Xiu-qin; Zeng, Chun-yan; Zhao, Li-li^*; Wan, Kang-lin^*

来源：Tuberculosis, 2017, 107: 1-4.

DOI：10.1016/j.tube.2017.07.010

• Multiplex allele specific PCR
• Multidrug-resistant tuberculosis
• Ofloxacin
• Kanamycin

Rapid detection of resistance to the second-line drugs is essential for early initiation of appropriate antitubercular treatment regimen among multi-drug tuberculosis (MDR-TB). In this study, we applied a multiplex allele-specific PCR (MAS-PCR) to identify the mutations on codons 90 and 94 of gyrA and nucleotide 1401 of rrs for detecting ofloxacin (OFX) and kanamycin (KAN) resistance in 139 MDR-TB isolates from China. Using the traditional phenotypic method as the reference, MAS-PCR detected resistance to OFX and KAN with sensitivities of 67.3% and 76.5%, respectively, and specificities of 100.0%. Therefore, MAS-PCR assays can be used for rapid detection of second-line drug resistance among MDR-TB in China, enabling early administration of appropriate treatment regimens to the affected MDR-TB patients.

• 出版日期2017-12
• 单位中国疾病预防控制中心传染病预防控制所; 传染病预防控制国家重点实验室; 首都医科大学; 北京老年医院