Disulfide-Containing Hyperbranched Polyethylenimine Derivatives via Click Chemistry for Nonviral Gene Delivery

作者：Jiang Xulin^*; Liu Jia; Xu Li; Zhuo Renxi

来源：Macromolecular Chemistry and Physics, 2011, 212(1): 64-71.

DOI：10.1002/macp.201000448

• biodegradable
• cross-linking
• hyperbranched
• intracellular gene delivery
• stimuli-sensitive polymers

Reducible disulfide-containing hyperbranched PEI-SS-HP was synthesized via click chemistry and evaluated as nonviral gene carrier. The structure of the polymers was confirmed by (1)H NMR and FTIR. It was shown that the PEI-SS-HP was able to bind plasmid DNA to positively charged nanoparticles. The reduction sensitivity of the PEI-SS-HP was confirmed by gel retardation assay in the presence of DTT mimicking an intracellular reductive environment. In vitro experiments revealed that the reducible PEI-SS-HP not only had much lower cytotoxicity, but also posed superior transfection activity as compared to non-degradable 25-kDa PEI. The results indicate that a reducibly degradable PEI-SS-HP can be a promising gene vector.

• 出版日期2011-1-4
• 单位武汉大学