Membrane-type 1 matrix metalloproteinase regulates fibronectin assembly to promote cell motility

作者：Takino Takahisa^*; Nagao Ryota; Manabe Ri ichiroh; Domoto Takahiro; Sekiguchi Kiyotoshi; Sato Hiroshi

来源：FEBS Letters, 2011, 585(21): 3378-3384.

DOI：10.1016/j.febslet.2011.09.039

• ECM
• Fibronectin
• Motility
• MT1-MMP
• Tumor

Fibronectin (FN) matrix assembly is an essential process in normal vertebrate development, which is frequently lost in tumor cells. Here we show that membrane-type 1 matrix metalloproteinase (MT1-MMP) regulates FN matrix assembly. MT1-MMP knockdown induced FN assembly in breast carcinoma cells. Ectopic expression of MT1-MMP reduced specifically the assembled FN matrix level without affecting whole FN production in fibroblasts. Treatment of fibrosarcoma HT1080 cells with dexamethasone (DEX) enhanced FN synthesis, resulting in short fibrils but not dense matrix formation. Combined treatment of DEX and MT1-MMP inhibitor accelerated FN matrix assembly, which mediated cellular adhesion and reduced cell migration and invasion. These results indicate that MT1-MMP stimulates cell migration and invasion by negatively regulating FN assembly.
Structured summary of protein interactions:
FN and Integrin Beta 1 colocalize by fluorescence microscopy (View

• 出版日期2011-11-4