SOS chromotest genotoxicity and toxicity of cyanobacterial extracts from reservoir surface water

作者：Bonislawska A^*; Demkowicz Dobrzanski K; Nalecz Jawecki G; Mankiewicz J; Tarczynska M; Sawicki J

来源：Fresenius Environmental Bulletin, 2003, 12(10): 1144-1151.

• cyanobacteria
• SOS Chromotest
• genotoxicity
• toxicity

The neurotoxins and hepatotoxins are the most dangerous cyanobacterial compounds. These poisons periodically contaminate the water bodies and drinking water. Information on the genotoxic effects of these compounds is ambiguous. In our present study the genotoxicity of cyanobacterial extracts was studied using SOS Chromotest. Cyanobacteria were homogenized and extracted using SEP PAK C18 or XAD-2 columns. The genotoxicity in the SOS Chromotest was expressed as induction factor (IF) or SOS-inducing potency (SOSIP). The induction of IF (up to 1.9) was observed when the cyanobacterial extract (CE) was tested without metabolic activation. The genotoxicity expressed as SOSIP correlated well with the contents of cyanobacterial toxins. The toxicity of CE expressed as inhibition of alkaline phosphatase activity also depended on toxin contents. CE was neither genotoxic nor toxic when it was incubated in vitro with S9 fraction from livers of male B10 A mice, pretreated with Aroclor 1254. The SOS Chromotest appears to be a convenient test system for routine screening of the genotoxicity and toxicity of cyanobacterial toxins.

• 出版日期2003