Assessment of microRNAs in patients with unstable angina pectoris

作者：Zeller Tanja; Keller Till; Ojeda Francisco; Reichlin Tobias; Twerenbold Raphael; Tzikas Stergios; Wild Philipp S; Reiter Miriam; Czyz Ewa; Lackner Karl J; Munzel Thomas; Mueller Christian; Blankenberg Stefan^*

来源：European Heart Journal, 2014, 35(31): 2106-2114.

DOI：10.1093/eurheartj/ehu151

• Circulating microRNA
• Myocardial infarction
• Unstable angina pectoris
• Acute myocardial infarction
• Diagnosis

Aims While cardiac troponin measurements have significantly improved the early diagnosis of myocardial infarction, the timely biomarker-based diagnosis of unstable angina pectoris (UAP) remains a major unmet clinical challenge. The aim of this study was to assess levels of circulating microRNAs (miRNAs) as possible novel biomarkers in patients with UAP. %26lt;br%26gt;Methods and results A three-phase approach was conducted, comprising (i) profiling of miRNAs in patients with UAP and controls groups; (ii) replication of significant miRNAs in an independent patient cohort, (iii) validation of a multi-miRNAs panel in a third cohort. Out of 25 miRNAs selected for replication, 8 miRNAs remained significantly associated with UAP. In a validation phase, a miRNA panel including miR-132, miR-150, and miR-186 showed the highest discriminatory power [area under the receiver-operating-characteristic curve (AUC): 0.91; CI: 0.84-0.98]. %26lt;br%26gt;Conclusion Using a profiling-replication-validation model, we identified eight miRNAs, which may facilitate the diagnosis of UAP.

• 出版日期2014-8-14