A myeloid differentiation factor 88 gene from yellow catfish Pelteobagrus fulvidraco and its molecular characterization in response to polyriboinosinic polyribocytidylic acid and lipopolysaccharide challenge

作者：Zhao, Xiao-Ming; Chu, Xiao-Hua; Liu, Yu; Liu, Qiu-Ning^*; Jiang, Sen-Hao; Zhang, Dai-Zhen; Tang, Bo-Ping^*; Zhou, Chun-Lin; Dai, Li-Shang^*

来源：International Journal of Biological Macromolecules, 2018, 120(Pt A): 1080-1086.

DOI：10.1016/j.ijbiomac.2018.08.189

• Myeloid differentiation factor 88
• Immune response
• Expression analysis

Myeloid differentiation factor 88 (MyD88) is an adaptor protein of Toll-like receptor (TLR) signalling pathways that activates the innate immune system. Herein, MyD88 was identified in the economically important freshwater fish Pelteobagrus fulvidraco. The complete 2156 bp PfMyD88 cDNA includes a 147 bp 5'-untranslated region (UTR), a 1133 bp 3'-UTR, and an open reading frame (ORF) of 876 bp encoding a 291 residue protein containing Death and Toll/interleukin-1 receptor (TIR) domains. The deduced protein sequence shares 88.8%, 73.8% and 59.3% identity with orthologs in Ictalurus punctatus, Danio rerio and Homo sapiens, respectively. qRT-PCR revealed expression in all tested tissues, highest in trunk kidney, followed by spleen, and lowest in muscle. After challenge with lipopolysaccharide (LPS) or polyriboinosinic polyribocytidylic acid (Poly l:C), PJMyD88 expression was up regulated in blood, liver, head kidney and spleen. Thus, P1MyD88 acts in innate immunity in P. fulvidmco.

• 出版日期2018-12
• 单位山西大学; 南京工业大学; 温州医科大学; 盐城师范学院