A rapid and sensitive method for the analysis of lignin phenols in environmental samples using ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry with multiple reaction monitoring

作者：Yan Ge; Kaiser Karl^*

来源：Analytica Chimica Acta, 2018, 1023: 74-80.

DOI：10.1016/j.aca.2018.03.054

• Lignin
• Ultra-high performance liquid
• chromatography
• Tandem mass spectrometry
• Gas chromatography
• C-13-labeled standards
• Solid-phase extraction

A novel ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method was developed to enable the analysis of ultra-low levels of lignin phenols (<100 fmol) in environmental samples. The method included a sample clean-up with a polymer sorbent based solid-phase extraction and reversed-phase chromatography that allowed separation of all lignin phenols with minimal interference from co-eluting matrix components. The application of C-13-labeled surrogate standards effectively compensated for variability associated with sample preparation and ion suppression. The method was fully validated and compared to gas chromatography-electron impact ionizationtandem mass spectrometry for Suwannee River humic acid standard reference material and extracted riverine dissolved organic matter. The comparison demonstrated the robustness and suitability of the method for ultra-low lignin phenol concentrations and high matrix interference. The simple and fast sample preparation coupled to analysis without derivatization makes it feasible for routine and high-volume analyses.

• 出版日期2018-9-6