A distinct tolerogenic subset of splenic IDO(+)CD11b(+) dendritic cells from orally tolerized mice is responsible for induction of systemic immune tolerance and suppression of collagen-induced arthritis

作者：Park Min Jung; Park Kyung Su; Park Hyun Sil; Cho Mi La; Hwang Sue Yun; Min So Youn; Park Mi Kyung; Park Sung Hwan; Kim Ho Youn^*

来源：Cellular Immunology, 2012, 278(1-2): 45-54.

DOI：10.1016/j.cellimm.2012.06.009

• Oral tolerance
• Indoleamine-2,3-dioxygenase
• Spleen
• Dendritic cell
• Regulatory T cell
• Collagen-induced arthritis

In oral tolerance, locally instigated tolerance in the gut propagate to systemic tolerance. In order to investigate the mechanism, we analyzed indoleamine 2,3-dioxygenase (IDO) expression in splenic dendritic cell (DC) subsets and tested whether DCs suppress collagen-induced arthritis (CIA) by inducing regulatory T cells (Tregs). The proportion of IDO-expressing cells was higher in the CD11b(+) subset of splenic DCs from orally tolerized CIA mice. These DCs suppressed type II collagen-specific T cell proliferation and promoted Treg induction from CD4(+)CD25(-)T cells using transforming growth factor-beta. These DCs also increased the expression of cytotoxic T lymphocyte antigen-4 and programmed death-1 on Tregs. When adoptively transferred, spenic IDO-expressing CD11b(+) DCs from tolerized animals suppressed the development of arthritis, increased the Treg/Th17 cell ratio, and decreased the production of inflammatory cytokines in the spleen. Taken together, a distinct subset of splenic IDO(+)CD11b(+)DCs is responsible for the systemic immune regulation in oral tolerance.

• 出版日期2012-8
• 单位河北医科大学