Assessment of four protocols for rapid bacterial identification from positive blood culture pellets by matrix-assisted laser desorption ionization-time of flight mass spectrometry (Vitek (R) MS)

作者：Thomin Jean; Aubin Guillaume Ghislain; Foubert Fabrice; Corvec Stephane^*

来源：Journal of Microbiological Methods, 2015, 115: 54-56.

DOI：10.1016/j.mimet.2015.05.010

• MALDI-TOF
• Blood bottle culture
• Rapid identification

In this study, we developed and compared four protocols to prepare a bacterial pellet from 944 positive blood cultures for direct MALDI-TOF mass spectrometry Vitek (R) MS analysis. Protocol 4, tested on 200 monomicrobial samples, allowed 83% of bacterial identification. This easy, fast, cheap and accurate method is promising in daily practice, especially to limit broad range antibiotic treatment.

• 出版日期2015-8