Development of F-19-NMR chemical shift detection of DNA B-Z equilibrium using F-19-NMR

作者：Nakamura S; Yang H; Hirata C; Kersaudy F; Fujimoto K^*

来源：Organic and Biomolecular Chemistry, 2017, 15(24): 5109-5111.

DOI：10.1039/c7ob00706j

Various DNA conformational changes are in correlation with biological events. In particular, DNA B-Z equilibrium showed a high correlation with translation and transcription. In this study, we developed a DNA probe containing 5-trifluoromethylcytidine or 5-trifluoromethylthymidine to detect DNA B-Z equilibrium using F-19-NMR. Its probe enabled the quantitative detection of B-, Z-, and ss-DNA based on F-19-NMR chemical shift change.

• 出版日期2017-6-28