Acute and chronic administration of gold nanoparticles cause DNA damage in the cerebral cortex of adult rats

作者：Cardoso Eria; Rezin Gislaine Tezza; Zanoni Elton Torres; Notoya Frederico de Souza; Leffa Daniela Dimer; Damiani Adriani Paganini; Daumann Francine; Rodriguez Juan Carlos Ortiz; Benavides Roberto; Da Silva Luciano; Andrade Vanessa M; Paula Marcos Marques da Silva^*

来源：Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 2014, 766: 25-30.

DOI：10.1016/j.mrfmmm.2014.05.009

• Gold nanoparticles
• DNA damage
• Comet assay
• Damage frequency
• Damage index
• Genotoxicity

The use of gold nanoparticles is increasing in medicine; however, their toxic effects remain to be elucidated. Studies show that gold nanoparticles can cross the blood-brain barrier, as well as accumulate in the brain. Therefore, this study was undertaken to better understand the effects of gold nanoparticles on rat brains. DNA damage parameters were evaluated in the cerebral cortex of adult rats submitted to acute and chronic administration of gold nanoparticles of two different diameters: 10 and 30 nm. During acute administration, adult rats received a single intraperitoneal injection of either gold nanoparticles or saline solution. During chronic administration, adult rats received a daily single injection for 28 days of the same gold nanoparticles or saline solution. Twenty-four hours after either single (acute) or last injection (chronic), the rats were euthanized by decapitation, their brains removed, and the cerebral cortices isolated for evaluation of DNA damage parameters. Our study showed that acute administration of gold nanoparticles in adult rats presented higher levels of damage frequency and damage index in their DNA compared to the control group. It was also observed that gold nanoparticles of 30 nm presented higher levels of damage frequency and damage index in the DNA compared to the 10 nm ones. When comparing the effects of chronic administration of gold nanoparticles of 10 and 30nm, we observed that occurred significant different index and frequency damage, comparing with control group. However, there is no difference between the 10 and 30 nm groups in the levels of DNA damage for both parameters of the Comet assay. Results suggest that gold nanoparticles for both sizes cause DNA damage for chronic as well as acute treatments, although a higher damage was observed for the chronic one.

• 出版日期2014-9