Distribution and binding of novel photosensitizer 2-devinyl-2-(1-methoxyl-ethyl) chlorin f in human breast cancer cells MCF-7

作者：Liu Y; Chen P^*; Zhang F; Lin L; Tang G Q; Mu G G

来源：Laser Physics Letters, 2009, 6(6): 465-471.

DOI：10.1002/lapl.200910017

• photosensitizers
• 2-devinyl-2-(1-methoxyl-ethyl) chlorin f (CPD4)
• human breast cancer cells MCF-7
• mitochondria
• cellular membrane

The interaction of novel Photosensitizer 2-devinyl-2-(1-methoxyl-ethyl) chlorin f (CPD4) with human breast cancer cells MCF-7 was studied by fluorescence spectrum and laser confocal scan microscopy (LCSM). The experimental results exhibit that fluorescence emission band of CPD4 in MCF-7 cells move to long wavelength about 5 nm compared with that in incubation solution, which suggest that CPD4 could enter into MCF-7 cells and bind them strongly by electrostatic or hydrogen binding interaction. The LCSM images of CPD4 in MCF-7 cells show that the binding of CPD4 in MCF-7 cells takes place in cellular membrane and mitochondria mainly. These results indicate that CPD4 can be of selective subcellular location, but not free ionic status in MCF-7 cells. CPD4 could be a kind of promising photosensitizer for Photodynamic therapy.
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LSCM images of int

• 出版日期2009-6
• 单位南开大学