Development of an Organic Lateral Resolution Test Device for Imaging Mass Spectrometry

作者：Passarelli Melissa K^*; Wang Jun; Mohammadi Amir Saeid; Trouillon Raphael; Gilmore Ian; Ewing Andrew G

来源：Analytical Chemistry, 2014, 86(19): 9473-9480.

DOI：10.1021/ac501228x

An organic lateral resolution test device has been developed to measure the performance of imaging mass spectrometry (IMS) systems. The device contains periodic gratings of polyethylene glycol (PEG) and lipid bars covering a wide range of spatial frequencies. Microfabrication technologies were employed to produce well-defined chemical interfaces, which allow lateral resolution to be assessed using the edge-spread ESF). In addition, the design of the device allows for the direct measurement of the modulation transfer MTF) to assess image quality. Scanning electron microscopy (SEM) and time-of-flight secondary ion mass spectrometry (TOF-SIMS) were used to characterize the device. TOF-SIMS imaging was used to measure the chemical displacement of biomolecules in matrix-assisted laser desorption/ionization (MALDI) matrix crystals. In a proof-of-concept experiment, the platform was also used to evaluate MALDI matrix application methods, specifically aerosol spray and sublimation methods.

• 出版日期2014-10-7