Development of a Calcium Phosphate Nanocomposite for Fast Fluorogenic Detection of Bacteria

作者：Martinez Claudio R; Rodriguez Tamara L; Zhurbenko Raisa; Valdes Ivonne A; Gontijo Savio M L; Gomes Alinne D M; Suarez Diego F; Sinisterra Ruben D; Cortes Maria E^*

来源：Molecules, 2014, 19(9): 13948-13964.

DOI：10.3390/molecules190913948

• hydroxyapatite
• bacterial adhesion
• nanoparticle
• nanocomposite
• fluorescence

Current procedures for the detection and identification of bacterial infections are laborious, time-consuming, and require a high workload and well-equipped laboratories. Therefore the work presented herein developed a simple, fast, and low cost method for bacterial detection based on hydroxyapatite nanoparticles with a nutritive mixture and the fluorogenic substrate. Calcium phosphate ceramic nanoparticles were characterized and integrated with a nutritive mixture for the early detection of bacteria by visual as well as fluorescence spectroscopy techniques. The composite was obtained by combining calcium phosphate nanoparticles (Ca:P ratio, 1.33:1) with a nutritive mixture of protein hydrolysates and carbon sources, which promote fast bacterial multiplication, and the fluorogenic substrate 4-methylumbellipheryl-beta-D-glucuronide (MUG). The composite had an average particle size of 173.2 nm and did not show antibacterial activity against Gram-negative or Gram-positive bacteria. After an Escherichia coli suspension was in contact with the composite for 60-90 min, fluorescence detected under UV light or by fluorescence

• 出版日期2014-9