Development of an array of ion-selective microelectrodes aimed for the monitoring of extracellular ionic activities

作者：Guenat Olivier T^*; Generelli Silvia; de Rooij Nicolaas F; Koudelka Hep Milena; Berthiaume Francois; Yarmush Martin L

来源：Analytical Chemistry, 2006, 78(21): 7453-7460.

DOI：10.1021/ac0609733

In this study, we present the development and the characterization of a generic platform for cell culture able to monitor extracellular ionic activities (K+, NH4+) for real-time monitoring of cell-based responses, such as necrosis, apoptosis, or differentiation. The platform for cell culture is equipped with an array of 16 silicon nitride micropipet-based ion-selective microelectrodes with a diameter of either 2 or 6 mu m. This array is located at the bottom of a 200-mu m- wide and 350-mu m-deep microwell where the cells are cultured. The characterization of the ion-selective microelectrode arrays in different standard and physiological solutions is presented. Near-Nernstian slopes were obtained for potassium- (58.6 +/- 0.8 mV/pK, n = 15) and ammonium-selective microelectrodes (59.4 +/- 3.9 mV/pNH(4), n = 13). The calibration curves were highly reproducible and showed an average drift of 4.4 +/- 2.3 mV/h (n = 10). Long-term behavior and response after immersion in physiological solutions are also presented. The lifetime of the sensors was found to be extremely long with a high recovery rate.

• 出版日期2006-11-1