科研之友
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摘要
Aquaporin (AQP) family plays a pivotal role in fluid secretion and absorption especially in the digestive system and secretory glands Within this family AQP8 was reported to be widely expressed in the epithelia of the digestive tract, liver, and pancreas In two parallel experimental platforms with different analytical and comparative approaches, in gel tryptic digestion with macro-embedded spreadsheet analysis and in-solution tryptic digestion with LC MS alignment based approach we compared wild type and AQP8 knockout mice colon proteomes Shared result between both experiments revealed down-regulation of alpha-amylase 2 in AQP8-deleted mice model Verification on both transcriptional and translational levels confirmed the involvement of AQP8 in alpha-amylase 2 regulation Given the profound role of AQP8 as a water and solutes transporter, it might be important in modulating a-amylase 2 synthesis by colonic epithelial cells as well Here, we also proved the capability of our coupled approaches for selecting the most reliable and significant candidates, an applicable process for initial screening of biological biomarkers in complex specimens and tissue extracts
- 出版日期2010-12
