Nylon Nanofibrous Biosensors for Glucose Determination

作者：Scampicchio Matteo; Arecchi Alessandra; Bianco Andrea; Bulbarello Andrea; Bertarelli Chiara; Mannino Saverio^*

来源：Electroanalysis, 2010, 22(10): 1056-1060.

DOI：10.1002/elan.200900446

• Electrospinning
• Nylon-6
• Glucose oxidase
• Nanofibrous membrane
• Amperometric detection
• Membranes
• Biosensors

Nanofibrous membranes have been produced by electrospinning to develop first generation glucose biosensors. The direct immobilization of glucose oxidase onto the polyamide nanofibrous surfaces by drop coating revealed a simple and efficient method for the development of sensitive, stable, and reproducible electrochemical biosensors. The biosensor showed a linear response over the range 1-9 x 10(-3) glucose (R(2) = 0.9997) with a sensitivity of 1.11 mu A/mM and a limit of detection of 2.5 x 10(-6) M (S/N = 3). The uncertainty of repeatability was 2% (RSD%, n = 30). After one month of storage, the signal decreased of 35%. The recovery of glucose, evaluated in real samples of honey, was 98% (RSD% = 1%, n = 3).

• 出版日期2010-5