Antibacterial and biofilm removal activity of a podoviridae Staphylococcus aureus bacteriophage SAP-2 and a derived recombinant cell-wall-degrading enzyme

作者：Son Jee Soo; Lee Se Jung; Jun Soo Youn; Yoon Seong Jun; Kang Sang Hyeon; Paik Hyoung Rok; Kang Jung Ok; Choi Yun Jaie^*

来源：Applied Microbiology and Biotechnology, 2010, 86(5): 1439-1449.

DOI：10.1007/s00253-009-2386-9

• Cell-wall-degrading enzyme
• Podoviridae
• Bacteriophage
• Biofilm
• Staphylococcus aureus

Antibacterial and biofilm removal activity of a new podoviridae Staphylococcus aureus bacteriophage (SAP-2), which belongs to the phi 29-like phage genus of the Podoviridae family, and a cell-wall-degrading enzyme (SAL-2), which is derived from bacteriophage SAP-2, have been characterized. The cell-wall-degrading enzyme SAL-2 was expressed in Escherichia coli in a soluble form using a low-temperature culture. The cell-wall-degrading enzyme SAL-2 had specific lytic activity against S. aureus, including methicillin-resistant strains, and showed a minimum inhibitory concentration of about 1 mu g/ml. In addition, this enzyme showed a broader spectrum of activity within the Staphylococcus genus compared with bacteriophage SAP-2 in its ability to remove the S. aureus biofilms. Thus, the cell-wall-degrading enzyme SAL-2 can be used to prevent and treat biofilm-associated S. aureus infections either on its own or in combination with other cell-wall-degrading enzymes with anti-S. aureus activity.

• 出版日期2010-5