Adrm1 interacts with Atp6v0d2 and regulates osteoclast differentiation

作者：Kim Taesoo; Ha Hyun Il; Kim Nacksung; Yi Okju; Lee Seoung Hoon^*; Choi Yongwon

来源：Biochemical and Biophysical Research Communications, 2009, 390(3): 585-590.

DOI：10.1016/j.bbrc.2009.10.010

• Osteoclast
• Atp6v0d2
• Adrm1
• Cell fusion
• Differentiation
• Yeast two-hybrid screen

Bone homeostasis is tightly regulated by matrix-producing osteoblasts and bone-resorbing osteoclasts. During osteoclast development, mononuclear preosteoclasts derived from myeloid cells fuse together to form multinucleated, giant cells. Previously, we reported that the d2 isoform of the vacuolar (H( )) ATPase V0 domain (Atp6v0d2) plays an important role in osteoclast maturation and bone formation To. understand how Atp6v0d2 Controls osteoclast maturation, we have performed a yeast two-hybrid screen using full-length Atp6v0d2 as the bait, and identified adhesion-regulating molecule 1 protein (Adrm1) as a potential functional partner of Atp6v0d2. The interaction between Atp6v0d2 and Adrm1 was confirmed in yeast and in vivo using immunoprecipitation assays. We also show that Adrm1 is required for cell migration and osteoclast maturation.

• 出版日期2009-12-18