科研之友
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摘要
Objectives: A sepsis rat model using the cecal ligation and puncture (CLP) was developed to study the histological alteration of the pancreas, and the pancreatic injury during the septic shock was evaluated. Methods: 36 healthy males specific-pathogen-free (SPF) Sprague-Dawley rats were divided into three groups: control group, shanm operative (SOP) group and sepsis group, with 6 rats in each. Sepsis group underwent CLP approach, while the cecum was freed from the mesentery in SOP group, except for CLP approach. The pancreatic tissue was removed from living rats at different time spot, and the morphology alteration of pancreas by light microscope and electron microscope was assessed. Results: A septic shock rat model was successfully established using CLP approach. Pancreas didn't show necrosis neither in control group nor SOP group at each time spot by light microscope. However, scattered acinar cells showed necrosis at six hours post CLP in sepsis group, while most acinar cells showed necrosis at twenty-four hours post CLP. While, a large number of mitochondria, lysosomes and endoplasmic reticulum showed swelling, expansion at twenty-four hours post CLP in sepsis group by election microscope, and diffuse vacuolar necrosis was found. Conclusion: Pancreatic damage may occur at the early stage of sepsis. Patients with sepsis might develop secondary pancreatic damage and should be aware of in clinic. The pathological alteration of pancreatic acinar cells would appear earlier than the mesenchyme. Timely intervention on the pancreas might help to reduce the mortality in critically ill patients with sepsis.
- 出版日期2017
- 单位广西医科大学
