Anti-HPA-15 alloimmunization: Prevalence and difficulties of the screening and characterization

作者：Moncharmont P^*; Bourgeot C; Merieux Y; Rigal D

来源：Immuno-Analyse & Biologie Specialisee, 2010, 25(1): 1-3.

DOI：10.1016/j.immbio.2009.10.004

• Platelet
• Alloimmunization
• HPA-15
• Screening
• Characterization

HPA-15a and -15b-specific alloantibodies (Ab) may induce refractoriness to platelet (PLT) transfusion and fetal neonatal alloimmune thrombocytopenia (FNAIT) due to maternal alloimmunization. In order to establish the prevalence of these Ab, a study was performed in non-selected patients with a "Monoclonal Antibody-specific Immobilization of Platelet Antigen"-adapted assay. Of 7000 samples, 279 were found initial reactive (IR) (4.0%). Five were not retested because of insufficient volume of serum. Among the 274 remaining samples, 20 were found to be repeat reactive (7.3%). After characterization, anti-HPA-15b were found in three sera, one of which from a patient with a severe FNAIT; 17 were anti-HPA-15a, however the interpretation of results was delicate with those. Anti-HPA-15Ab reactivity depends on the type of PLT used and on the level of expression of the antigens present on the PLT membrane. HPA-15-specific Ab are uncommon and their detection remains difficult in practice.

• 出版日期2010-2