Activations of TRPA1 and P2X receptors are important in ROS-mediated stimulation of capsaicin-sensitive lung vagal afferents by cigarette smoke in rats

作者：Lin You Shuei; Hsu Chun Chun; Bien Mauo Ying; Hsu Hui Chi; Weng Hsu Ting; Kou Yu Ru^*

来源：Journal of Applied Physiology, 2010, 108(5): 1293-1303.

DOI：10.1152/japplphysiol.01048.2009

• lung vagal C fibers
• reactive oxygen species

Lin YS, Hsu CC, Bien MY, Hsu HC, Weng HT, Kou YR. Activations of TRPA1 and P2X receptors are important in ROS-mediated stimulation of capsaicin-sensitive lung vagal afferents by cigarette smoke in rats. J Appl Physiol 108: 1293-1303, 2010. First published February 18, 2010; doi:10.1152/japplphysiol.01048.2009.-Capsaicin-sensitive lung vagal afferents (CSLVAs) are important in detecting pulmonary reactive oxygen species (ROS). We investigated the mechanisms underlying the stimulation of CSLVAs by inhaled cigarette smoke (CS) in 216 anesthetized rats. In spontaneously breathing rats, CS evoked a CSLVA-mediated reflex bradypnea that was prevented by N-acetyl-L-cysteine (NAC; an antioxidant), HC-030031 [a transient receptor potential ankyrin 1 (TRPA1) receptor antagonist], and iso-pyridoxalphosphate-6-azophenyl-2', 5'-disulfonate (iso-PPADS; a P2X receptor antagonist). In paralyzed, artificially ventilated rats, CS evoked an increase in CSLVA fiber activity (Delta FA) that was abolished by NAC and was attenuated by HC-030031, iso-PPADS, indomethacin (Indo; a cyclooxygenase inhibitor), and a combination of apyrase and adenosine deaminase (ADA) (ATP scavengers); the response to CS was reduced to 11.7 +/- 4.0%, 39.5 +/- 10.0%, 52.9 +/- 14.4%, 68.7 +/- 10.1%, and 47.2 +/- 12.9% of control, respectively. The suppressive effect on this afferent response was not improved by a combination of HC-030031 and Indo (Delta FA = 39.5 +/- 10.1% of control) compared with that induced by HC-030031 alone. In contrast, the suppressive effect was enhanced by a combination of HC-030031 and apyrase + ADA (Delta FA = 5.3 +/- 4.9% of control) or a combination of iso-PPADS and Indo (Delta FA = 23.3 +/- 7.7% of control) compared with that induced by HC-030031 alone or iso-PPADS alone. This afferent response was not altered by the vehicles for these drugs. These results suggest that activations of TRPA1 receptors by cyclooxygenase metabolites and P2X receptors by ATP are both necessary for the ROS-mediated stimulation of CSLVA fibers by CS in rats.

• 出版日期2010-5
• 单位河北医科大学