High resolution melting PCR to differentiate Mycobacterium avium subsp paratuberculosis"cattle type" and "sheep type"

作者：Douarre Pierre E; Cashman William; Buckley Jim; Coffey Aidan; O'Mahony Jim M^*

来源：Journal of Microbiological Methods, 2012, 88(1): 172-174.

DOI：10.1016/j.mimet.2011.10.009

• Mycobacterium avium subsp paratuberculosis
• Genotyping
• PCR
• High resolution melting

In this study, we describe a novel HRM-PCR assay that clearly differentiates the two main types of Mycobacterium avium subsp. paratuberculosis (cattle and sheep) based on the polymorphic variation of a previously described tandem repeat. This modern genotyping technique has several advantages over alternative methods, including cost, ease of use and rapidity.

• 出版日期2012-1