A noncoding antisense RNA in tie-1 locus regulates tie-1 function in vivo

作者：Li Keguo; Blum Yannick; Verma Anjali; Liu Zhong; Pramanik Kallal; Leigh Noah R; Chun Chang Z; Samant Ganesh V; Zhao Baofeng; Garnaas Maija K; Horswill Mark A; Stanhope Stephen A; North Paula E; Miao Robert Q; Wilkinson George A; Affolter Markus; Ramchandran Ramani^*

来源：Blood, 2010, 115(1): 133-139.

DOI：10.1182/blood-2009-09-242180

Recently, messenger RNAs in eukaryotes have shown to associate with antisense (AS) transcript partners that are often referred to as long noncoding RNAs (IncRNAs) whose function is largely unknown. Here, we have identified a natural AS transcript for tyrosine kinase containing immunoglobulin and epidermal growth factor homology domain-1 (tie-1), tie-1AS IncRNA in zebrafish, mouse, and humans. In embryonic zebrafish, tie-1AS IncRNA transcript is expressed temporally and spatially in vivo with its native target, the tie-1 coding transcript and in additional locations (ear and brain). The tie-1AS IncRNA selectively binds tie-1 mRNA in vivo and regulates tie-1 transcript levels, resulting in specific defects in endothelial cell contact junctions in vivo and in vitro. The ratio of tie-1 versus tie-1AS IncRNA is altered in human vascular anomaly samples. These results directly implicate noncoding RNA-mediated transcriptional regulation of gene expression as a fundamental control mechanism for physiologic processes, such as vascular development. (Blood. 2010;115:133-139)

• 出版日期2010-1-7