Nitric oxide releasing material adsorbs more fibrinogen

作者：Lantvit Sarah M; Barrett Brittany J; Reynolds Melissa M^*

来源：Journal of Biomedical Materials Research Part A, 2013, 101(11): 3201-3210.

DOI：10.1002/jbm.a.34627

• adsorption
• fibrinogen
• poly(vinyl chloride)
• nitric oxide
• surface analysis

One mechanism of the failure of blood-contacting devices is clotting. Nitric oxide (NO) releasing materials are seen as a viable solution to the mediation of surface clotting by preventing platelet activation; however, NO%26apos;s involvement in preventing clot formation extends beyond controlling platelet function. In this study, we evaluate NO%26apos;s effect on factor XII (fibrinogen) adsorption and activation, which causes the initiation of the intrinsic arm of the coagulation cascade. This is done by utilizing a model plasticized poly(vinyl) chloride (PVC), N-diazeniumdiolate system and looking at the adsorption of fibrinogen, an important clotting protein, to these surfaces. The materials have been prepared in such a way to eliminate changes in surface properties between the control (plasticized PVC) and composite (NO-releasing) materials. This allows us to isolate NO release and determine the effect on the adsorption of fibrinogen, to the material surface. Surprisingly, it was found that an NO releasing material with a surface flux of 17.4 +/- 0.5 x 10(-10) mol NO cm(-2) min(-1) showed a significant increase in the amount of fibrinogen adsorbed to the material surface compared to one with a flux of 13.0 +/- 1.6 x 10(-10) mol NO cm(-2) min(-1) and the control (2334 +/- 496, 226 +/- 99, and 103 +/- 31% fibrinogen adsorbed of control, respectively). This study suggests that NO%26apos;s role in controlling clotting is extended beyond platelet activation.

• 出版日期2013-11