Molecular and functional characterization of a new 3 ' end KIT juxtamembrane deletion in a duodenal GIST treated with neoadjuvant Imatinib

作者：Perfetti Vittorio; Laurini Erik; Aulic Suzana; Fermeglia Maurizio; Riboni Roberta; Lucioni Marco; Dallera Elena; Delfanti Sara; Pugliese Luigi; Latteri Francesco Saverio; Pietrabissa Andrea; Pricl Sabrina^*

来源：Oncotarget, 2017, 8(34): 56158-56167.

DOI：10.18632/oncotarget.19341

• KIT
• GIST
• novel deletion mutation
• Imatinib
• neoadjuvant treatment

Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the gastrointestinal tract. GISTs express the receptor tyrosine kinase KIT, and the majority of GISTs present KIT gain-of-function mutations that cluster in the 5' end of the receptor juxtamembrane domain. On the other hand, little information is known about GISTs carrying mutations in the 3' end of the KIT juxtamembrane domain. Here we report and discuss a clinical case of localized duodenal GIST whose molecular characterization revealed the presence of a new 21 nucleotide/7 amino acid deletion in the 3' end of KIT juxtamembrane domain (Delta 574-580). The patient was treated with Imatinib at standard regimen dose (400 mg/day), and responded well as the original tumor mass reduced, ultimately allowing conservative surgery. In line with these clinical evidences computer simulations, biophysical techniques and in vitro experiments demonstrated that the receptor tyrosine kinase KIT carrying the Delta 574-580 mutation displays constitutive phosphorylation, which can be switched-off upon Imatinib treatment. In addition, results from this study showed that a clinical useful procedure, neoadjuvant treatment, can occasionally be of value for the understanding of the molecular pathogenesis of GIST.

• 出版日期2017-8-22