A novel alkaline serine protease with fibrinolytic activity from the polychaete, Neanthes japonica

作者：Wang Shaohua; Deng Zhihui; Li Qi; Ge Xin; Bo Qiqing; Liu Jiankai; Cui Jiayue; Jiang Xi; Liu Jia; Zhang Lianzhi; Hong Min^*

来源：Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 2011, 159(1): 18-25.

DOI：10.1016/j.cbpb.2011.01.004

• Alkaline serine protease
• Fibrinogenolytic activity
• Fibrinolytic activity
• Neanthes japonica (Izuka)
• Purification

A new protease named NJP with fibrinolytic activity was isolated from Neanthes japonica (Izuka), by a combination of ammonium sulfate fractionation, hydrophobic chromatography, ion-exchange chromatography and gel filtration. The molecular mass of NJP was approximately 28.6-33.5 kDa as estimated by MALDI-TOF mass spectrometry and SDS-PAGE. which revealed a monomeric form of the protease. The isoelectric point of NJP determined by 2-DE was 9.2. NJP was stable in the range of pH 7.0-11.0 with a maximum enzymatic activity at 40 degrees C and pH 9.0. The hydrolyzing activity of NJP on fibrinogen started from the A alpha-chain, followed by the B beta-chain, and the gamma-chain at last. NJP had also a higher specificity for the chromogenic substrate S-2238 for thrombin. NJP activity was completely inhibited by PMSF. Analysis of partial amino acid sequences showed that NJP had very low homology with other known fibrinolytic enzymes. These results indicate that NJP is a novel alkaline thrombin-like serine protease. Thus NJP may have potential applications in the prevention and treatment of thrombosis.

• 出版日期2011-5
• 单位吉林大学; 齐齐哈尔医学院