A Eukaryotic Expression Plasmid Carrying Chicken Interleukin-18 Enhances the Response to Newcastle Disease Virus Vaccine

作者：Wang, Chen^*; Li, Xiaokang; Zhang, Chunjie; Wu, Tingcai; Li, Yinju; Cheng, Xiangchao

来源：Clinical and Vaccine Immunology, 2015, 22(1): 56-64.

DOI：10.1128/CVI.00636-14

• CELLULAR IMMUNE-RESPONSES
• IFN-GAMMA PRODUCTION
• CD4(+) T-CELLS
• DNA VACCINE
• B-CELLS
• ADJUVANT ACTIVITY
• INTERFERON-GAMMA
• IL-18
• TH1
• INDUCTION

Interleukin-18 (IL-18) is an important cytokine involved in innate and acquired immunity. In this study, we cloned the full-length chicken IL-18 (ChIL-18) gene from specific-pathogen-free (SPF) chicken embryo spleen cells and provided evidence that the ChIL-18 gene in a recombinant plasmid was successfully expressed in chicken DT40 cells. ChIL-18 significantly enhanced gamma interferon (IFN-gamma) mRNA expression in chicken splenocytes, which increased IFN-gamma-induced nitric oxide (NO) synthesis by macrophages. The potential genetic adjuvant activity of the ChIL-18 plasmid was examined in chickens by coinjecting ChIL-18 plasmid and inactivated Newcastle disease virus (NDV) vaccine. ChIL-18 markedly elevated serum hemagglutination inhibition (HI) titers and anti-hemagglutinin-neuraminidase (anti-HN)-specific antibody levels, induced the secretion of both Th1-(IFN-gamma) and Th2-(interleukin-4) type cytokines, promoted the proliferation of T and B lymphocytes, and increased the populations of CD3(+) T cells and their subsets, CD3(+) CD4(+) and CD3(+) CD8(+) T cells. Furthermore, a virus challenge revealed that ChIL-18 contributed to protection against Newcastle disease virus challenge. Taken together, our data indicate that the co-administration of ChIL-18 plasmid and NDV vaccine induces a strong immune response at both the humoral and cellular levels and that ChIL-18 is a novel immunoadjuvant suitable for NDV vaccination.

• 出版日期2015-1
• 单位河南大学