NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

作者：Lee Ronald F S; Escrig Stephane; Croisier Marie; Clerc Rosset Stephanie; Knott Graham W; Meibom Anders; Davey Curt A; Johnsson Kai; Dyson Paul J^*

来源：Chemical communications, 2015, 51(92): 16486-16489.

DOI：10.1039/c5cc06983a

The in vitro inter-and intra-cellular distribution of an isotopically labelled ruthenium(II)-arene (RAPTA) anti-metastatic compound in human ovarian cancer cells was imaged using nano-scale secondary ion mass spectrometry (NanoSIMS). Ultra-high resolution isotopic images of C-13, N-15, and Ru indicate that the phosphine ligand remains coordinated to the ruthenium(II) ion whereas the arene detaches. The complex localizes mainly on the membrane or at the interface between cells which correlates with its anti-metastatic effects.

• 出版日期2015