Upregulation of Intestinal Glucose Transporters after Roux-en-Y Gastric Bypass to Prevent Carbohydrate Malabsorption

作者：Nguyen Nam Q^*; Debreceni Tamara L; Bambrick Jenna E; Chia Bridgette; Deane Adam M; Wittert Gary; Rayner Chris K; Horowitz Michael; Young Richard L

来源：Obesity, 2014, 22(10): 2164-2171.

DOI：10.1002/oby.20829

ObjectiveTo determine the effect of Roux-en-Y gastric bypass (RYGB) on the expression of intestinal sweet taste receptors (STRs), glucose transporters (GTs), glucose absorption, and glycemia. %26lt;br%26gt;MethodsIntestinal biopsies were collected for mRNA expression of STR (T1R2) and GTs (SGLT-1 and GLUT2) from 11 non-diabetic RYGB, 13 non-diabetic obese, and 11 healthy subjects, at baseline and following a 30 min small intestinal (SI) glucose infusion (30 g/150 ml water with 3 g 3-O-methyl-d-glucopyranose (3-OMG)). Blood glucose, plasma 3-OMG, and insulin were measured for 270 min. %26lt;br%26gt;ResultsIn RYGB patients, expression of both GTs was approximate to 2-fold higher at baseline and after glucose infusion than those of morbidly obese or healthy subjects (P%26lt;0.001). STR expressions were comparable amongst the groups. Peak plasma 3-OMG in both RYGB (r=0.69, P=0.01) and obese (r=0.72, P=0.005) correlated with baseline expression of SGLT-1, as was the case with peak blood glucose in RYGB subjects (r=0.69, P=0.02). %26lt;br%26gt;ConclusionsThe upregulated intestinal GTs in RYGB patients are associated with increased glucose absorption when glucose is delivered at a physiological rate, suggesting a molecular adaptation to prevent carbohydrate malabsorption from rapid intestinal transit after RYGB.

• 出版日期2014-10