Asymmetrical flow field-flow fractionation with multi-angle light scattering and quasi elastic light scattering for characterization of poly(ethyleneglycol-b-epsilon-caprolactone) block copolymer self-assemblies used as drug carriers for photodynamic therapy

作者：Ehrhart Jerome; Mingotaud Anne Francoise; Violleau Frederic^*

来源：Journal of Chromatography A, 2011, 1218(27): 4249-4256.

DOI：10.1016/j.chroma.2011.01.048

• Asymmetrical flow field-flow fractionation
• Static light scattering
• Dynamic light scattering
• Nanoparticles
• Self-assembly
• Drug delivery

Poly(ethyleneoxide-b-epsilon-caprolactone) (PEO-b-PCL) self-assemblies in water were characterized by asymmetrical flow field-flow fractionation (AsFIFFF), with on-line coupling with quasi-elastic light scattering (QELS), multi-angle light scattering (MALS), refractive index and UV/Vis detection. We report here the AsFIFFF analysis of three different nanoparticular self-assembled systems of PEO-PCL polymers in aqueous media, each polymer differing by the mass of the PEO and PCL fragments. Thus, self-assembled water samples of {PEO(2000)-b-PCL(2600)}, {PEO(5000)-b-PCL(1400)} and {PEO(5000)-b-PCL(4000)} were analyzed by AsFIFFF. In most cases, the size obtained by AsFIFFF was similar to the one characterized by DLS. However, in some instances, only AsFIFFF revealed the presence of several self-assemblies with very different sizes. These nanoparticles being used for the targeted delivery of photosensitizers in photodynamic therapy, it was important to fully characterize the samples in terms of size and size distribution, molecular weight, lp, aggregation number and also to assess whether the photosensitizer was inside the nanoparticles. AsFIFFF proved to be a very efficient technique which enabled this study without any destruction of the nanoparticles.

• 出版日期2011-7-8