Differentiation of human endometrial stem cells into endothelial-like cells on gelatin/chitosan/bioglass nanofibrous scaffolds

作者：Shamosi Atefeh; Mehrabani Davood; Azami Mahmoud; Ebrahimi Barough Somayeh; Siavashi Vahid; Ghanbari Hossein; Sharifi Esmaeel; Roozafzoon Reza; Ai Jafar

来源：Artificial Cells, Nanomedicine and Biotechnology, 2017, 45(1): 163-173.

DOI：10.3109/21691401.2016.1138493

• Endothelial cells
• gelatin/chitosan/bioglass
• human endometrial stem cells
• nanofiber scaffold

The capacity of gelatin/chitosan/bioactive glass nanopowders (GEL/CS/BGNPs) scaffolds was investigated for increasing human endometrial stem cells (hEnSCs) differentiation into the endothelial cells in the presence of angiogenic factors. GEL/CS nanofibrous scaffold with different contents of BGNPs were fabricated and assessed. Expression of endothelial markers (CD31, vascular endothelial cadherin (VE-cadherin), and KDR) in differentiated cells was evaluated. Results showed the diameter of nanofiber increases with decreasing the BG content in GEL/CS scaffolds. Moreover, in vitro study indicated that the GEL/CS/BGNPs scaffold with 1.5% BGNPs content provided a suitable three-dimensional structure for endothelial cells differentiation. Thus, the GEL/CS/BGNPs scaffold can be recommended for blood vessels repair.

• 出版日期2017-2