Space mutagenesis of genetically engineered bacteria expressing recombinant human interferon alpha 1b and screening of higher yielding strains

作者：Wang, Junfeng; Liu, Changting^*; Liu, Jinyi; Fang, Xiangqun; Xu, Chen; Guo, Yinghua; Chang, De; Su, Longxiang

来源：World Journal of Microbiology and Biotechnology, 2014, 30(3): 943-949.

DOI：10.1007/s11274-013-1512-0

• Space mutagenesis
• Genetically engineered bacteria
• Recombinant human interferon alpha 1b
• Screening

The aim of this study was to investigate the space mutagenesis of genetically engineered bacteria expressing recombinant human interferon alpha 1b. The genetically engineered bacteria expressing the recombinant interferon alpha 1b were sent into outer space on the Chinese Shenzhou VIII spacecraft. After the 17 day space flight, mutant strains that highly expressed the target gene were identified. After a series of screening of spaceflight-treated bacteria and the quantitative comparison of the mutant strains and original strain, we found five strains that showed a significantly higher production of target proteins, compared with the original strain. Our results support the notion that the outer space environment has unique effects on the mutation breeding of microorganisms, including genetically engineered strains. Mutant strains that highly express the target protein could be obtained through spaceflight-induced mutagenesis.

• 出版日期2014-3
• 单位中国人民解放军总医院