Acetylome Analysis Identifies SIRT1 Targets in mRNA-Processing and Chromatin-Remodeling in Mouse Liver

作者：Kim, Sun-Yee; Sim, Choon Kiat; Tang, Hui; Han, Weiping; Zhang, Kangling^*; Xu, Feng

来源：PLos One, 2015, 10(10): e0140619.

DOI：10.1371/journal.pone.0140619

• HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS
• LYSINE ACETYLATION
• METABOLISM
• SIRTUINS
• GLUCOSE
• ROLES
• INFLAMMATION
• DEACETYLASES
• RESVERATROL
• PGC-1-ALPHA

Lysine acetylation is a post-translational modification found on numerous proteins, a strategy used in cell signaling to change protein activity in response to internal or external cues. Sirtuin 1 (SIRT1) is a central lysine deacetylase involved in a variety of cellular processes including metabolism, apoptosis, and DNA repair. Here we characterize the lysine acetylome in mouse liver, and by using a model of Sirt1(-/-) knockout mouse, show that SIRT1 regulates the deacetylation of 70 proteins in the liver in-vivo. Amongst these SIRT1-regulated proteins, we find that four RNA-processing proteins and a chromatin-remodeling protein can be deacetylated by SIRT1 directly in-vitro. The discovery that SIRT1 has a potential role in RNA-processing suggests a new layer of regulation in the variety of functions performed by SIRT1.

• 出版日期2015-10-15