Measurement of Atropisomer Racemization Kinetics Using Segmented Flow Technology

作者：Davoren Jennifer E^*; Bundesmann Mark W; Yan Qi T; Collantes Elizabeth M; Mente Scot; Nason Deane M; Gray David L

来源：ACS Medicinal Chemistry Letters, 2012, 3(5): 433-435.

DOI：10.1021/ml2003108

• Atropisomer
• Reaction kinetics
• Segmented flow chemistry

When stable atropisomers are encountered by drug discovery teams, they can have important implications due to potential differences in their biological activity, pharmacokinetics, and toxicity. Knowledge of an atropisomer's activation parameters for interconversion is required to facilitate informed decisions on how to proceed. Herein, we communicate the development of a new method for the rapid measurement of atropisomer racemization kinetics utilizing segmented flow technology. This method leverages the speed, accuracy, low sample requirement, safety, and semiautomated nature of flow instrumentation to facilitate the acquisition of kinetics data required for experimentally probing atropisomer activation parameters. Measured kinetics data obtained for the atropo isomerization of AMPA antagonist CP-465021 using segmented flow and traditional thermal methods were compared to validate the method.

• 出版日期2012-5